Application of Flow Cytometry for Viability Assessment of Mutants for Translation Termination Factors in the Yeast Saccharomyces cerevisiae-Reference-Cited by-同舟云学术

Application of Flow Cytometry for Viability Assessment of Mutants for Translation Termination Factors in the Yeast Saccharomyces cerevisiae

Published:2024-08-28 Issue:2 Volume:93 Page:239-243
ISSN:0026-3656
Container-title:Микробиология
language:
Short-container-title:Microbiology

Author:

Efremova E. P.¹,Zemlyanko O. M.¹²,Zhouravleva G. A.¹²

Affiliation:

1. St. Petersburg State University

2. Laboratory of Amyloid Biology SPBU

Abstract

Nonsense mutations in the essential SUP45 and SUP35 genes, encoding translation termination factors, affect the viability of Saccharomyces cerevisiae cells. Flow cytometry revealed that the viability of mutants was 3.5‒4 times lower compared to the wild-type. Moreover, the mutants were found to have higher sensitivity to ultrasonic treatment.

Publisher

The Russian Academy of Sciences

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