Analysis of the Key Determinants of Naphthalene Degradation by Rhodococcus pyridinivorans 5Ар-Reference-Cited by-同舟云学术

Analysis of the Key Determinants of Naphthalene Degradation by Rhodococcus pyridinivorans 5Ар

Published:2023-07-01 Issue:4 Volume:92 Page:418-426
ISSN:0026-3656
Container-title:Микробиология
language:
Short-container-title:Microbiology

Author:

Larchenka A. Yu.¹,Mandryk M. I.¹

Affiliation:

1. Belarusian State University

Abstract

Abstract—Wild-type cells of Rhodococcus pyridinivorans 5Ар were found to be highly efficient naphthalene degraders, completely utilizing this compound (500 mg/L) after 3 days, and may be used for remediation of naphthalene-contaminated aquatic ecosystems. Inactivation of the biodegradation genes narAa (encoding the large subunit of naphthalene dioxygenase) and narB (encoding cis-naphthalene dihydrodiol dehydrogenase) resulted it the loss of ability to use naphthalene as the sole energy source, which indicated the absence in the genome of R. pyridinivorans 5Ар of the determinants responsible for alternative pathways of naphthalene oxidation. Moreover, narB inactivation resulted in accumulation of a polar colored compound (probably a product of primary naphthalene oxidation) in the medium.

Publisher

The Russian Academy of Sciences

Reference29 articles.

1. Каталог штаммов региональной профилированной коллекции алканотрофных микроорганизмов / Под ред. Ившиной И.Б.. М.: Наука, 1994.

2. Catalog of Strains of the Regional Profiled Collection of Alkanotrophic Microorganisms / Ed. Ivshina I.B. M.: Nauka, 1994.

3. Маниатис Т., Фрич Э., Сэмбрук Дж. Методы генетической инженерии. Молекулярное клонирование / Пер. с англ. под ред.Баева А.А., Скрябина К.Г. М.: Мир, 1984. 479 с. Maniatis T., Fritsch E.F., Sambrook J. Molecular Cloning. A Laboratory Manual. New York: Cold Spring Harbor Laboratory, 1982. 545 p.

4. Нафталин коксохимический. Технические условия: ГОСТ 16106-2019. введ. 01.03.20. Межгосударственный совет по стандартизации, метрологии и сертификации, 2019. 12 с. Расимович Б.Э. Биодеградация нефтезагрязнений под воздействием углеводородокисляющих микроорганизмов // НефтеГазоХимия. 2019. № 1. С. 48–51.

5. Романенко В.И., Кузнецов С.И. Экология микроорганизмов пресных вод. М.: Наука, 1974. 194 с.