Metagenomic Analysis of Bottom Sediments of the Karst Meromictic Lake Black Kichier Revealed Abundant Unculturable Thermoplasmatota

Author:

Kadnikov V. V.1,Savvichev A. S.2,Rusanov I. I.2,Beletskii A. V.1,Pimenov N. V.2

Affiliation:

1. Skryabin Institute of Bioengineering, Research Centre of Biotechnology, Russian Academy of Sciences

2. Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences

Abstract

Investigations into the microbial community of bottom sediments in the karst lake Black Kichier were conducted. These sediments exhibited elevated levels of sulfide, dissolved methane, and organic matter. Direct radiotracer experiments revealed substantial rates of microbial processes involved in the decomposition of organic matter. Uncultivated archaea belonging to the phylum Thermoplasmatota were identified within the microbial community. Metagenomic analysis unveiled representatives from five orders: Methanomassiliicoccales, Thermoprofundales (formerly known as Marine Benthic Group D and DHVEG-1), DTX01, SG8-5, and Candidatus Gimiplasmatales (formerly UBA10834). These archaea were previously believed to occur exclusively in deep marine sediments characterized by extreme organic matter scarcity. This discovery reshapes our understanding of the role played by Thermoplasmatota archaea, spanning five orders, in the degradation segment of the carbon cycle.

Publisher

The Russian Academy of Sciences

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