Polymorphism of microsatellite loci in populations of Caucasian rock lizards and its use for assessing the genetic diversity of Darevskia raddei

Abstract

The study of Caucasian rock lizards of the Darevskia raddei complex, represented by several subspecies, is of great interest and scientific significance in connection with their participation in interspecific hybridizations with the formation of five of the seven known unisexual (parthenogenetic) species of the genus Darevskia. Here are presented genetic parameters for populations (subspecies) of D. r. raddei and D. r. nairensis based on the analysis of the variability of 10 microsatellite loci of 230 individuals from 17 populations of Armenia and Nagorno-Karabakh. According to these parameters, D. r. raddei are characterized by greater diversity in the number of alleles and genotypes compared to D. r. nairensis. Genetic differentiation analysis showed that D. r. raddei populations are divided into two groups, one of which is genetically closer to D. r. nairensis than D. r. raddei. Analysis of the association index showed the absence of free recombination of alleles between subspecies, which indicates their isolation and the absence of crossing between individuals. Thus, based on the expanded population sample and the developed panel of microsatellite markers, new data on the population structure of D. raddei species, genetic diversity and differentiation of D. r. raddei and D. r. nairensis were obtained.