Epr study of the generation of short-lived reactive oxygen species in rat heart homogenate

Author:

Timoshin A. A1,Lakomkin V. L1,Abramov A. A1,Ruuge E. K12

Affiliation:

1. Chazov National Medical Research Centre for Cardiology of the Ministry of Health of the Russian Federation

2. Lomonosov Moscow State University

Abstract

The aim of the present study was to investigate the protective effects of a mitochondrial-targeted antioxidant such as plastomitin through ROS hypergeneration in animals. The average rate of the generation of highly reactive oxygen radicals during oxidative stress, induced by doxorubicin injection in rats and a protective impact of plastomitin use were investigated. The experiments were carried out with rat heart tissue homogenates using EPR spectroscopy. It was found that in case of doxorubicin injection in rats, there is a significant 26% increase in the rate of short-lived reactive oxygen species generation in the homogenate but when doxorubicin and plastomitin were used in combination, this effect was greatly suppressed.

Publisher

The Russian Academy of Sciences

Reference9 articles.

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3. В. Л. Лакомкин и В. И. Капелько, Кардиология, 49 (10), 55 (2009).

4. M. A. Mitry and J. G. Edwards, IJC Heart & Vasculature, 10, 17 (2016).

5. H. Nohl, L. Gille, and K. Staniek, Zeitschrift Fur Naturforschung. C, 53 (3-4), 279 (1998).

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