Quantitative determination of natural rubber content of Taraxacum kok-saghyz E. Rodin plants using spin probe method of electron paramagnetic resonance spectroscopy

Author:

Martirosyan L. Yu12,Goldberg V. M1,Barashkova I. I2,Kasparov V. V1,Martirosyan Yu. Ts12,Motyakin M. V13,Gaydamaka S. N4,Varfolomeev S. D14

Affiliation:

1. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

2. All-Russian Research Institute of Agricultural Biotechnology

3. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences

4. Lomonosov Moscow State University

Abstract

The paper presents a method to carry out a quantitative analysis of the content of natural rubber of Taraxacum kok-saghyz E. Rodin by measuring adsorption of the nitroxide radical TEMPO in root preparations of the said plant. The method is based on a comparison between the integrated intensity of an EPR signal of interest and a standard. The developed method of analysis makes it possible to quickly and with good accuracy determine the content of rubber of plant tissues without its extraction from rubber-containing biomass.

Publisher

The Russian Academy of Sciences

Reference20 articles.

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4. Goodyear to develop domestic source of natural rubber, https://www.prnewswire.com/news-releases/good-year-to-develop-domestic-source-of-natural-rubber-301519783.html

5. A. Y. Amerik, et al., Rus. J. Plant Physiol., 68 (1), 31 (2021).

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