Affiliation:
1. Federal Scientific Rice Centre
Abstract
This article presents the results of studies on the determination of informative DNA marker systems that provide reliable control of the presence of Xcc-loci of resistance to black rot in the breeding material of white cabbage. At the initial stage of the work, 20 molecular markers taken from the VegMarks database were tested on isogenic cabbage lines contrasting in resistance to black rot (resistant line 269-Yas12p-2 and susceptible line Pi714). It was found that only the SSR marker Ol10-C01 reveals polymorphism between contrasting samples of white cabbage. PCR analysis with the use of this polymorphic marker and phytopathological testing have been also performed on F2 plants of the hybrid combination 269-Yas12p-2 × Pi714. As a result of the statistical analysis of cleavage, it was found that the SSR marker Ol10-C01 is co-inherited with a trait of resistance to black rot, since the expected segregation of F2 plants by genotype 1:2:1 according to Mendel’s law by this locus and the optimal frequency of recombination between the Xcc resistance locus and the marker (13.7%) are observed.
Publisher
The Russian Academy of Sciences
Reference15 articles.
1. Batin N.V. Komp’uterniy statisticheskiy analiz dannykh. Minsk, 2008. 160 s.
2. Koroleva S.V., Dyakunchak S.A., Sitnikov S.V. Immunologicheskaya otsenka selektsionnogo materiala pri sozdanii gibridov F1 belokochannoy kapusty s gruppovoy ustoychivost’yu k fusariozu i sosudistomu bakteriozu (metodicheskie rekomendatsii). M., 2012. 16 s.
3. Koroleva S.V., Dyakunchak S.A., Yurchenko S.A. Development of F1 hybrids of cabbage with complex resistance in the south of Russia. Vegetable crops of Russia. 2019; 4: 16–20. https://doi.org/10.18619/2072-9146-2019-4-16-20
4. Kutlunina N.A., Ermoshin A.A. Molekulyarno-geneticheskie metody v issledovanii rasteniy. Ekaterinburg, 2017. 142 s.
5. Nguen M.L., Monakhos G.F., Komakhin R.A., Monakhos S.G. New resistance locus to clubroot in chromosome A05 of Chinese cabbage (Brassica rapa L.) // Rus. J. Genetics. 2018. V. 54. № 3. P. 306–315.