Investigation of the Possibilities of Analysis of the Vesicular Structure of PTNS-Based Nanodrugs Using the Small-Angle Neutron Scattering Data

Abstract

The small-angle neutron scattering spectra of polydispersed populations of unilamellar vesicles were analyzed depending on the concentration of maltose in a heavy water solution. The spectra were measured on a YuMO small-angle spectrometer at the Dzhelepov Laboratory of Neutron Physics of the Joint Institute for Nuclear Research (Dubna). Two types of nanosystems developed at the Orekhovich Institute of Biomedical Chemistry. This is a phospholipid transport nanosystem, as well as the Indolip nanodrug based on a phospholipid transport nanosystem. The possibilities of obtaining information on the vesicular structure of nanodrugs based on a phospholipid transport nanosystem using the small-angle neutron scattering data are discussed. Computer analysis of small-angle scattering spectra is based on the application of the separated form factors method. The values of the basic structural parameters of these vesicular systems (the average radius of vesicles in the population, the thickness of the bilayer across the membrane, the polydispersity coefficient, etc.) obtained on this basis are generally consistent with the corresponding results of similar processing of small-angle X-ray scattering data. However, compared with small-angle X-ray scattering, the method of small-angle neutron scattering turns out to be less sensitive to the detailed consideration of structural features of the vesicle shell bilayer.