Alzheimer's disease DNA methylome of pyramidal layers in frontal cortex: laser-assisted microdissection study-Reference-Cited by-同舟云学术

Alzheimer's disease DNA methylome of pyramidal layers in frontal cortex: laser-assisted microdissection study

Published:2018-11 Issue:11 Volume:10 Page:1365-1382
ISSN:1750-1911
Container-title:Epigenomics
language:en
Short-container-title:Epigenomics

Author:

Hernández Hernán Guillermo¹²,Sandoval-Hernández Adrián Gabriel³⁴,Garrido-Gil Pablo⁵⁶,Labandeira-Garcia José Luis⁵⁶,Zelaya María Victoria⁷,Bayon Gustavo F⁸,Fernández Agustín F⁹,Fraga Mario F¹⁰,Arboleda Gonzalo³⁴,Arboleda Humberto³

Affiliation:

1. PhD Program in Dentistry, Universidad Santo Tomás, Bucaramanga, Colombia

2. Research Unity, Universidad Manuela Beltrán, Bucaramanga, Colombia

3. Grupo de Neurociencias y muerte Celular, Facultad de Medicina e instituto de Genética, Universidad Nacional de Colombia, Colombia

4. Área de Bioquímica, Departamento de Química Universidad Nacional de Colombia, Colombia

5. Laboratory of Neuroanatomy and Experimental Neurology, Department of Morphological Sciences, CIMUS, Faculty of Medicine, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain

6. Networking Research Center on Neurodegenerative Diseases (CIBERNED), Madrid, Spain

7. Navarrabiomed Brain Bank, Navarra Institute for Health Research, Pamplona, Navarra, Spain

8. Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Hospital Universitario Central de Asturias (HUCA), Universidad de Oviedo, Principado de Asturias, Spain

9. Fundación para la Investigación Biosanitaria de Asturias (FINBA), Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Principado de Asturias, Spain

10. Nanomaterials and Nanotechnology Research Center (CINN-CSIC), Universidad de Oviedo, Principado de Asturias, Spain

Abstract

Objective: To study DNA methylation patterns of cortical pyramidal layers susceptible to late-onset Alzheimer's disease (LOAD) neurodegeneration. Methods: Laser-assisted microdissection to select pyramidal layers’ cells in frontal cortex of 32 human brains (18 LOAD) and Infinium DNA Methylation 450K analysis were performed to find differential methylated positions and regions, in addition to the corresponding gene set functional enrichment analyses. Results: Differential hypermethylation in several genomic regions and genes mainly in HOXA3, GSTP1, CXXC1-3 and BIN1. The functional enrichment analysis revealed genes significantly related to oxidative-stress and synapsis. Conclusion: The present results indicate the differentially methylated genes related to neural projections, synapsis, oxidative stress and epigenetic regulator genes and represent the first epigenome of cortical pyramidal layers in LOAD.

Publisher

Future Medicine Ltd

Subject

Cancer Research,Genetics

Link

https://www.futuremedicine.com/doi/pdf/10.2217/epi-2017-0160

Reference96 articles.

1. Alzheimer's Disease

2. Genetics of Alzheimer’s Disease in Caribbean Hispanic and African American Populations

3. Toward a Comprehensive Theory for Alzheimer's Disease. Hypothesis: Alzheimer's Disease Is Caused by the Cerebral Accumulation and Cytotoxicity of Amyloid β-Protein

4. The Amyloid Hypothesis of Alzheimer's Disease: Progress and Problems on the Road to Therapeutics

5. Role of Genes and Environments for Explaining Alzheimer Disease

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