Epigenome-wide analysis of DNA methylation reveals a rectal cancer-specific epigenomic signature-Reference-Cited by-同舟云学术

Epigenome-wide analysis of DNA methylation reveals a rectal cancer-specific epigenomic signature

Published:2016-09 Issue:9 Volume:8 Page:1193-1207
ISSN:1750-1911
Container-title:Epigenomics
language:en
Short-container-title:Epigenomics

Author:

Vymetalkova Veronika¹²,Vodicka Pavel¹²³,Pardini Barbara⁴,Rosa Fabio⁴,Levy Miroslav⁵,Schneiderova Michaela⁶,Liska Vaclav³⁷,Vodickova Ludmila¹²³,Nilsson Torbjörn K,Farkas Sanja A⁸

Affiliation:

1. Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic

2. Institute of Biology & Medical Genetics, 1st Medical Faculty, Charles University, Prague, Czech Republic

3. Biomedical Centre, Faculty of Medicine in Pilsen, Charles University, Czech Republic

4. Human Genetics Foundation, (HuGeF), Torino, Italy

5. Department of Surgery, 1st Faculty of Medicine, Charles University & Thomayer Hospital, Prague, Czech Republic

6. Department of Surgery, General University Hospital, Prague, Czech Republic

7. Department of Surgery, Teaching Hospital & Medical School in Pilsen, Charles University, Pilsen, Czech Republic

8. Department of Laboratory Medicine, Örebro University; Örebro, Sweden

Abstract

Aim: The aim of the present study is to address a genome-wide search for novel methylation biomarkers in the rectal cancer (RC), as only scarce information on methylation profile is available. Materials & methods: We analyzed methylation status in 25 pairs of RC and adjacent healthy mucosa using the Illumina Human Methylation 450 BeadChip. Results: We found significantly aberrant methylation in 33 genes. After validation of our results by pyrosequencing, we found a good agreement with our findings. The BPIL3 and HBBP1 genes resulted hypomethylated in RC, whereas TIFPI2, ADHFE1, FLI1 and TLX1 were hypermethylated. An external validation by TCGA datasets confirmed the results. Conclusion: Our study, with external validation, has demonstrated the feasibility of using specific methylated DNA signatures for developing biomarkers in RC.

Publisher

Future Medicine Ltd

Subject

Cancer Research,Genetics

Link

https://www.futuremedicine.com/doi/pdf/10.2217/epi-2016-0044

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