Virus membrane proteins and proteinaceous pores

Abstract

Enveloped viruses penetrate the host cells by fusion of the viral envelope with a cellular target membrane. One of the best studied viruses with respect to its penetration and uncoating is the alphavirus Semliki Forest virus that is taken up by endocytosis. The alphavirus membrane glycoprotein E1 harbors a so-called fusion peptide, which is responsible for interaction with the endosomal membrane, leading to fusion. Besides this fusion process, cell infection by alphaviruses is accompanied by membrane permeability changes, thus implying some form of pore across the membrane. However, the ability of E1 protein to form ion pores has not been widely accepted. This review provides an overview of studies that confirm earlier results predicting the formation of a proteinaceous pore by the alphavirus spike proteins. Furthermore, different models to explain this pore formation during virus entry are discussed.