Comprehensive data analysis for development of custom qRT-PCR miRNA assay for glioblastoma: a prevalidation study

Author:

Toraih Eman A12,Abdallah Hoda Y12,Rashed Essam A34,El-Wazir Aya12,Tantawy Mohamed A5,Fawzy Manal S67

Affiliation:

1. Department of Histology & Cell Biology, Genetics Unit, Faculty of Medicine, Suez Canal University, Ismailia 41522, Egypt

2. Center of Excellence of Molecular & Cellular Medicine, Suez Canal University, Ismailia, Egypt

3. Department of Mathematics, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt

4. Department of Computer Science, Faculty of Informatics and Computer Science, The British University in Egypt, Cairo 11837, Egypt

5. Hormones Department, Medical Research Division, National Research Center, Cairo, Egypt

6. Department of Medical Biochemistry & Molecular Biology, Faculty of Medicine, Suez Canal University, Ismailia 41522, Egypt

7. Department of Biochemistry, Faculty of Medicine, Northern Border University, Arar, Saudi Arabia

Abstract

Aim: Glioblastoma (GB) is one notable example of miRNA-modulated neoplasms. Given its unique expression signature, proper miRNA profiling can help discriminate between GB and other types of brain tumors. The current work aimed to develop a more GB-specific and applicable custom designed quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) miRNA assay. Materials & methods: A comprehensive data analysis of bioinformatics databases, previous literature and commercially available pre-designed miRNA PCR arrays within the market. Results: A highly enriched panel of 84 deregulated and GB-specific miRNAs has been developed. Conclusion: After validation of this newly developed array, it can not only save the researcher's time and effort, but can also have a potential diagnostic and/or prognostic role in GB, paving the road toward personalized medicine.

Publisher

Future Medicine Ltd

Subject

Cancer Research,Genetics

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