Bioinformatic analysis of miRNA–mRNA interaction associated with LMP2A gene in nasopharyngeal carcinoma

Abstract

Aim: The purpose of the study was to construct miRNA–mRNA network associated with LMP2A in nasopharyngeal carcinoma (NPC). Materials & methods: The dataset GSE53914, GSE12452 and GSE26596 were downloaded from Gene Expression Omnibus and differentially expressed genes (DEGs) identified by GEO2R. Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed by ClusterProfiler R package. Protein–protein interaction network and mRNA–miRNA network associated with LMP2A were constructed. Hub genes were identified by Cytoscape. Results: The 135 DEGs associated with LMP2A were identified in NPC. Gene ontology function analysis showed DEGs were significantly enriched in cell–cell adhesion and NF-κB pathway. The hub genes were related to cell cycle. miRNA–mRNA network associated with LMP2A was constructed. Conclusion: The network may provide a way to explore the function of LMP2A in NPC by miRNA.