Methylation status of <i>VTRNA2-1</i>/<i>nc886</i> is stable across populations, monozygotic twin pairs and in majority of tissues-Reference-Cited by-同舟云学术

Methylation status of VTRNA2-1/nc886 is stable across populations, monozygotic twin pairs and in majority of tissues

Published:2022-09 Issue:18 Volume:14 Page:1105-1124
ISSN:1750-1911
Container-title:Epigenomics
language:en
Short-container-title:Epigenomics

Author:

Marttila Saara¹²^ORCID,Tamminen Hely¹,Rajić Sonja¹,Mishra Pashupati P³⁴⁵,Lehtimäki Terho³⁴⁵,Raitakari Olli⁶⁷⁸,Kähönen Mika⁴⁹,Kananen Laura¹⁰¹¹¹²,Jylhävä Juulia¹¹¹²,Hägg Sara¹¹,Delerue Thomas¹³,Peters Annette¹⁴¹⁵,Waldenberger Melanie¹³¹⁵,Kleber Marcus E¹⁶¹⁷,März Winfried¹⁶¹⁸¹⁹²⁰,Luoto Riitta²¹²²,Raitanen Jani²²²³,Sillanpää Elina²⁴²⁵,Laakkonen Eija K²⁴,Heikkinen Aino²⁵,Ollikainen Miina²⁵,Raitoharju Emma¹⁴^ORCID

Affiliation:

1. Molecular Epidemiology, Faculty of Medicine & Health Technology, Tampere University, Arvo Ylpön katu 34, Tampere, 33520, Finland

2. Gerontology Research Center, Tampere University, Tampere, 33014, Finland

3. Department of Clinical Chemistry, Faculty of Medicine & Health Technology, Tampere University, Arvo Ylpön katu 34, Tampere, 33520, Finland

4. Finnish Cardiovascular Research Center Tampere, Faculty of Medicine & Health Technology, Tampere University, Arvo Ylpön katu 34, Tampere, 33520, Finland

5. Fimlab Laboratories, Arvo Ylpön katu 4, Tampere, 33520, Finland

6. Centre for Population Health Research, University of Turku & Turku University Hospital, Turku, 20014, Finland

7. Research Centre of Applied & Preventive Cardiovascular Medicine, University of Turku, Turku, 20014, Finland

8. Department of Clinical Physiology & Nuclear Medicine, Turku University Hospital, Turku, 20014, Finland

9. Department of Clinical Physiology, Tampere University Hospital, Tampere, 33521, Finland

10. Faculty of Medicine & Health Technology, & Gerontology Research Center, Tampere University, Arvo Ylpön katu 34, Tampere, 33520,Finland

11. Department of Medical Epidemiology & Biostatistics, Karolinska Institutet, Stockholm, 171 77, Sweden

12. Faculty of Social Sciences (Health Sciences), & Gerontology Research Center, Tampere University, Arvo Ylpön katu 34, Tampere, 33520, Finland

13. Research Unit Molecular Epidemiology, Institute of Epidemiology, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Bavaria, D-85764, Germany

14. Institute of Epidemiology, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Bavaria, D-85764, Germany

15. German Center for Cardiovascular Research (DZHK), Partner Site Munich Heart Alliance, Munich, Germany

16. Vth Department of Medicine, Medical Faculty Mannheim, Heidelberg University, Mannheim, 68167, Germany

17. SYNLAB MVZ Humangenetik Mannheim, Mannheim, Germany

18. Competence Cluster for Nutrition & Cardiovascular Health (nutriCARD) Halle-Jena-Leipzig, Jena, 07743, Germany

19. SYNLAB Academy, SYNLAB Holding Deutschland GmbH, Augsburg, 86156, Germany

20. Clinical Institute of Medical & Chemical Laboratory Diagnostics, Medical University of Graz, Graz, 8010, Austria

21. The Social Insurance Institute of Finland (Kela), Helsinki, 00250, Finland

22. The UKK Institute for Health Promotion Research, Kaupinpuistonkatu 1, Tampere, 33500, Finland

23. Faculty of Social Sciences (Health Sciences), Tampere University, Arvo Ylpön katu 34, Tampere, 33520, Finland

24. Gerontology Research Center & Faculty of Sport & Health Sciences, University of Jyväskylä, Jyväskylä, 40014, Finland

25. Institute for Molecular Medicine Finland, FIMM, HiLIFE, University of Helsinki, Helsinki, 00014, Finland

Abstract

Aims & methods: The aim of this study was to characterize the methylation level of a polymorphically imprinted gene, VTRNA2-1/ nc886, in human populations and somatic tissues.48 datasets, consisting of more than 30 tissues and >30,000 individuals, were used. Results: nc886 methylation status is associated with twin status and ethnic background, but the variation between populations is limited. Monozygotic twin pairs present concordant methylation, whereas ∼30% of dizygotic twin pairs present discordant methylation in the nc886 locus. The methylation levels of nc886 are uniform across somatic tissues, except in cerebellum and skeletal muscle. Conclusion: The nc886 imprint may be established in the oocyte, and, after implantation, the methylation status is stable, excluding a few specific tissues.

Funder

Yrjö Jahnssonin Säätiö

Päivikki and Sakari Sohlberg foundation

Laboratoriolääketieteen edistämissäätiö sr

Swedish Research Council

Signe ja Ane Gyllenbergin Säätiö

Suomen Akatemia

the Sigrid Juselius Foundation

the Tampere University Hospital Medical Funds

H2020 European Research Council

Pirkanmaa Regional Fund of Finnish Cultural Foundation

Karolinska Institutet Strategic Research Program in Epidemiology