Comprehensive analysis of differentially expressed long noncoding RNAs, miRNAs and mRNAs in breast cancer brain metastasis

Author:

An Meng1,Zang Xiaowen2,Wang Jimin1,Kang Jie3,Tan Xiaoyu1,Fu Bo4ORCID

Affiliation:

1. Department of Clinical Laboratory, Liaocheng People’s Hospital, Liaocheng, PR China

2. Department of Neurology First Ward, Liaocheng Veterans Hospital, Liaocheng, PR China

3. Department of Stomatology, Liaocheng People’s Hospital, Liaocheng, PR China

4. Department of Central Laboratory, Liaocheng People’s Hospital, Medical College of Liaocheng University, Liaocheng, PR China

Abstract

Aim: To delineate the transcriptomic landscape and potential molecular mechanisms of breast cancer brain metastasis (BCBM). Materials & methods: Whole-transcriptome sequencing was performed to identify long noncoding RNA (lncRNA), miRNA and mRNA expression profiles associated with BCBM. Results: A total of 739 differentially expressed lncRNAs, 115 differentially expressed miRNAs and 5749 differentially expressed mRNAs were identified in 231-BR cells compared with MDA-MB-231 cells. Real-time quantitative PCR results revealed the expression levels of candidate molecules were consistent with their correspondence RNA-seq data. Protein–protein interaction analysis identified some hub genes associated with BCBM, such as PTBP1, NUP98 and HYOU1. LncRNA-miRNA-mRNA network highlighted a potential mechanism of BCBM in which lncRNA FIRRE and RP11-169F17.1 sponging hsa-miR-501-5p to regulate the expression of MMS19, PTBP1 and NUP98. Conclusion: This study provides a framework for better understanding molecular mechanisms of BCBM.

Funder

China Postdoctoral Science Foundation

Medicine and Health Science Technology Foundation of Shandong Province

National Nature Science Foundation of China

Nature Science Foundation of Shandong Province

Science Foundation of Liaocheng People's Hospital

Publisher

Future Medicine Ltd

Subject

Cancer Research,Genetics

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