HBx induces expression of CTGF in the transfected hepatoma cell line HepG2

Abstract

Aim: To investigate the effect of HBx on CTGF expression by hepatocytes. Materials & methods: HepG2 cells were transfected with the full-length gene of HBV, HBV protein-expressing plasmids, rhTGFβ1, LY2109761 or Smad2 siRNA, respectively, using Lipofectamine 3000. CTGF expression was detected by real-time PCR, ELISA, respectively. Then the effect of IL-32 on CTGF promoter was assayed by the Dual Luciferase® Reporter Assay System. Results: We found that HBx could induce CTGF expression by HepG2 cells in a concentration-dependent manner. CTGF expression induced by HBx employed the activation of TGFβ1-Smad2 signal pathway. Inhibition of TGFβ1 or Smad2 decreased CTGF expression induced by HBx. Conclusion: HBV might be involved in the pathogenesis of liver fibrosis through the HBx-induced CTGF expression.