Co-existence of NDM-, aminoglycoside- and fluoroquinolone-resistant genes in carbapenem-resistant <i>Escherichia coli</i> clinical isolates from Pakistan-Reference-Cited by-同舟云学术

Co-existence of NDM-, aminoglycoside- and fluoroquinolone-resistant genes in carbapenem-resistant Escherichia coli clinical isolates from Pakistan

Published:2023-09 Issue:14 Volume:18 Page:959-969
ISSN:1746-0913
Container-title:Future Microbiology
language:en
Short-container-title:Future Microbiology

Author:

Qamar Muhammad Usman¹^ORCID,Ejaz Hasan²^ORCID,Mohsin Mashkoor³,Hadjadj Linda⁴,Karadeniz Aylin⁴,Rolain Jean-Marc⁴,Saleem Zikria⁵^ORCID,Diene Seydina M⁴

Affiliation:

1. Institute of Microbiology, Faculty of Life Sciences, Government College University Faisalabad, 38000, Pakistan

2. Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Saudi Arabia

3. Institute of Microbiology, University of Agriculture, Faisalabad, 38000, Pakistan

4. Aix Marseille Univ, IRD, APHM, MEPHI, IHU-Méditerranée Infection, Faculté de Pharmacie, Marseille, France

5. Department of Pharmacy Practice, Faculty of Pharmacy, Bahauddin Zakariya University, Multan, 60800, Pakistan

Abstract

Background: To determine the prevalence of antimicrobial-resistant genes in carbapenem-resistant Escherichia coli (CRECO). Methods: A total of 290 carbapenem-resistant bacteria were collected from tertiary care hospitals in Lahore (Pakistan). These isolates were confirmed by VITEK 2 and matrix-assisted laser desorption/ionization time of flight. The minimum inhibitory concentration was performed by VITEK 2. Sequence typing, resistant gene identification, DNA hybridization and replicate typing were also performed. Results: 33 out of 290 (11.3%) were CRECO and carried blaNDM; 69, 18 and 12% were NDM-1, NDM-5 and NDM-7, respectively, with 100% resistance to β-lactams and β-lactam inhibitors. ST405 and ST468 were mostly identified. NDM-ECO carried approximately 50–450 kb of plasmids and 16 (55%) were associated with IncA/C. Conclusion: NDM-1-producing E. coli are highly prevalent in clinical settings.

Publisher

Future Medicine Ltd

Subject

Microbiology (medical),Microbiology

Link

https://www.futuremedicine.com/doi/pdf/10.2217/fmb-2023-0068

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