Long-term durability, tissue regeneration and neo-organ growth during skeletal maturation with a neo-bladder augmentation construct

Abstract

Aims: To comparatively evaluate bladder regeneration following 80% cystectomy and augmentation using a synthetic biopolymer with autologous urothelial and smooth muscle cells (autologous neo-bladder augmentation construct [construct]) or autotransplantation of native bladder (reimplanted native urinary bladder [reimplant]) in canines. Materials & methods: Voiding function, urodynamic assessment and neo-organ capacity-to-body-weight ratio (C:BW) were assessed longitudinally for a total of 24 months following trigone-sparing augmentation cystoplasty in juvenile canines. Results: Within 30 days postimplantation, hematology and urinalysis returned to baseline. Constructs and reimplants yielded neo-organs with statistically equivalent urodynamics and histology. Linear regression analysis of C:BW showed that constructs regained baseline slope and continued to adapt with animal growth. Conclusions: Constructs and reimplants regained and maintained native bladder histology by 3 months, capacity at 3–6 months and compliance by 12–24 months. Furthermore, construct C:BW demonstrated the ability of regenerated bladder to respond to growth regulation.