Phytochemical characterization and biological activities of Plectranthus barbatus Andrews

Author:

Cordeiro M. F.1ORCID,Nunes T. R. S.2ORCID,Bezerra F. G.2ORCID,Damasco P. K. M.2ORCID,Silva W. A. V.2ORCID,Ferreira M. R. A.2ORCID,Magalhães O. M. C.2ORCID,Soares L. A. L.2ORCID,Cavalcanti I. M. F.2ORCID,Pitta M. G. R.2ORCID,Rêgo M. J. B. M.2ORCID

Affiliation:

1. Universidade Federal de Pernambuco, Brasil; Universidade Federal do Vale do São Francisco, Brasil

2. Universidade Federal de Pernambuco, Brasil

Abstract

Abstract Plectranthus barbatus Andrews (Lamiaceae) is widely distributed in the world and has a range of popular therapeutic indications. This work aimed to evaluate the phytochemical characterization of two leaf extracts of P. barbatus, and their antimicrobial, antineoplastic and immunomodulatory potential. After collection, herborization and obtainment of the P. barbatus aqueous extract (PBA) and acetone:water 7:3 P. barbatus organic extract (PBO), the phytochemical characterization was performed by high-performance liquid chromatography (HPLC). The antimicrobial activity was performed to determine the minimum inhibitory concentration (MIC) against eight bacterial strains using the microdilution test and the fungus Trichophyton rubrum by disc diffusion assay and microdilution test. Cytotoxicity was assessed by MTT and trypan blue methods in normal peripheral blood mononuclear cells (PBMCs) at concentrations ranged between 0.1 to 100 µg.mL-1 and in neoplastic cell lines Toledo, K562, DU-145 and PANC-1 at 1, 10 and 100 µg.mL-1 . Immunomodulatory activity, was evaluated by sandwich ELISA of proinflammatory cytokines at BALB/c mice splenocytes cultures supernatant. Both extracts presented flavonoids, cinnamic derivatives, steroids and ellagic acid. PBO showed bacteriostatic activity against Acinetobacter baumannii (MIC = 250 µg.mL-1) clinical isolate and PBA fungistatic activity against Trichophyton rubrum (MIC = 800 µg.mL-1). The extracts did not exhibit toxicity to PBMCs and neoplastic cells (IC50 > 100 µg.mL-1). Additionally, PBO at 100 µg.mL-1 significantly inhibited IFN-γ and IL-17A cytokines (p = 0.03). Plectranthus barbatus is a potential candidate for therapeutic use due to its low toxicity in healthy human cells and exhibits biological activities of medical interest as bacteriostatic, fungistatic and immunomodulatory.

Publisher

FapUNIFESP (SciELO)

Subject

General Agricultural and Biological Sciences

Reference46 articles.

1. Forskolin: a potential antimetastatic agent;AGARWAL K.C.;International Journal of Cancer,1983

2. Therapeutic potential of flavonoids and their mechanism of action against microbial and viral Infections-a review;AHMAD A.;Food Research International,2015

3. Plectranthus barbatus: a review of phytochemistry, ethnobotanical uses and pharmacology – Part 1;ALASBAHI R.H.;Planta Medica,2010

4. General overview of phenolics from plant to laboratory, good antibacterials or Not;ALDULAIMI O.A.;Pharmacognosy Reviews,2017

5. Atividade antimicrobiana de extratos e de compostos fenólicos nitrogenados de café: avaliação in vitro e em modelo alimentar.;ALMEIDA A.A.P.,2007

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