Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system-Reference-Cited by-同舟云学术

Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system

Published:2006-07 Issue:4 Volume:49 Page:547-555
ISSN:1516-8913
Container-title:Brazilian Archives of Biology and Technology
language:
Short-container-title:Braz. arch. biol. technol.

Author:

Bezerra Raquel Pedrosa¹,Borba Fernanda Katharine Sousa Lins¹,Moreira Keila Aparecida²,Lima-Filho José Luis³,Porto Ana Lúcia Figueiredo⁴,Chaves Adilson Castro⁵

Affiliation:

1. Universidade de Pernambuco, Brasil; Universidade Federal de Pernambuco, Brasil

2. Universidade Federal de Pernambuco, Brasil; Universidade Federal de Pernambuco, Brasil

3. Universidade Federal de Pernambuco, Brasil

4. Universidade Federal de Pernambuco, Brasil; Universidade Federal Rural de Pernambuco, Brasil

5. Universidade de Pernambuco, Brasil; Universidade Federal de Pernambuco, Brasil; Faculdade de Formação de Professores de Nazaré da Mata, Brasil; Fundação de Ensino Superior de Olinda, Brasil; Universidade de Pernambuco, Brasil

Abstract

Studies were carried out on the partition of amylase from Bacillus subtilis in a minimal medium at 37 ºC and 110 rpm. Enzyme recovery was carried out in aqueous two-phase system PEG-Phosphate salt were carried out. The best purification factor (5.4) was obtained in system PEG 1000 (16.7% w/w) with potassium phosphate (14.8% w/w), at pH 6.0, resulting in a recovery of 45.2% activity enzymatic in the salt-rich phase.

Publisher

FapUNIFESP (SciELO)

Subject

Multidisciplinary

Link

http://www.scielo.br/pdf/babt/v49n4/30893.pdf

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