Temporal genotypic diversity of Schizaphis graminum (Rondani 1852) (Hemiptera: Aphididae) in a black oats (Avena strigosa) field

Abstract

The aim of this work was to analyze the clonal diversity variation in Schizaphis graminum during a complete phenological cycle of black oats (Avena strigosa). RAPD markers were used for detection of DNA polymorphisms of each clonal lineage, derived from a single clone collected weekly during a period of four months, in a crop field of black oats, Londrina, Paraná, Brazil. The monthly genotypic diversity was estimated by Shannon Information Index (H). Only four genotypes were distinguished from 122 specimens, with one of them overly predominant in all sampling dates (>60%). Another genotype, apparently a later colonizer, rapidly reached greater frequency than other genotypes on the second and third month. The results of this work suggested that temporal genotypic diversity of S. graminum assessed by RAPD markers was small and less variable than the genetic variation found at geographical scale.