Affiliation:
1. Universidade Federal do Rio de Janeiro, Brazil
2. Universidade do Estado do Rio de Janeiro, Brazil
Abstract
ABSTRACT Irrigation water and cultivated soil have been identified as possible sources of contamination in several crops. In certain vegetables that are eaten raw, such as lettuce, this contamination can lead to public health problems. Aiming to evaluate the influence of these sources on the quality of lettuce grown in the Córrego Sujo Basin, Teresópolis, RJ, an important agricultural pole whose production services the metropolitan region of Rio de Janeiro, water from different sources (spring, weir and river) was collected in this region, as well as samples of soil and lettuce irrigated with these waters, to carry out conventional microbiological analyzes (counts of total heterotrophic bacteria and thermotolerant coliforms) and molecular analyzes (PCR-DGGE). The count of fecal coliforms in lettuce suggests that there is an influence of irrigation water and the cultivated soil on the contamination of these vegetables. The grouping of bacterial communities in the different samples obtained by the PCR-DGGE technique shows that irrigation water has a greater influence on the contamination of these vegetables in relation to the soil where they are grown. These results corroborate the need to monitor water bodies used for irrigation and demonstrate that the PCR-DGGE technique is of great value for the study of microbial communities and, when associated with specific primers, can help in the detection of pathogens in food.
Subject
Waste Management and Disposal
Reference34 articles.
1. Microbiological quality and productivity of lettuce under chemical and organic fertilization;ABREU I.M.O.;Ciência e Tecnologia de Alimentos,2010
2. Factors affecting the efficacy of washing procedures used in the production of prepared salads;ADAMS M.R.;Food Microbiology,1989
3. Detection of Staphylococcus aureus in milk by PCR;AHMADI M.;Comparative Clinical Pathology,2010
4. Resolução RDC nº 12, de 2 de janeiro de 2001,2001
5. Irrigation Water Quality for Leafy Crops: A Perspective of Risks and Potential Solutions;ALLENDE A.;International Journal of Environmental Research and Public Health,2015
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