Affiliation:
1. Embrapa Recursos Genéticos e Biotecnologia Parque Estação Biológica, Brasil
Abstract
In this review, the use of the most common selection marker genes on plant transformation and the effects of their respective selective agents are discussed. These genes could be divided in two categories according their mode of action: genes for positive and negative selection. The retention of the marker gene flow through chloroplast transformation is also discussed. Further, strategies to recover marker-free transgenic plants, involving multi-auto-transformation (MAT), co-transformation, site-specific recombination and intragenomic relocation of transgenes through transposable elements are reviewed.
Subject
Plant Science,Agronomy and Crop Science
Cited by
17 articles.
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