Affiliation:
1. Fundação Oswaldo Cruz; Universidade Federal de Pernambuco
Abstract
Dacron and nitrocellulose were evaluated as matrices for the dot enzyme linked immunosorbent assay (dot-ELISA) for schistosomiasis and compared to indirect immunofluorescence (IMF). Titration of sera from 18 schistosomiasis patients against soluble worm antigen preparation (SWAP) was carried out and sera from healthy individuals from non-endemic areas were used as controls. The IMF was less sensitive than the dot-ELISAs, although the difference was not statistically significant (p > 0.05). The dot-ELISA based on nitrocellulose was as sensitive as that using dacron. Stability did not differ between nitrocellulose and dacron. Specificity was lower when dacron was used than when nitrocellulose was used, although the difference was not statistically significant (p > 0.05). In conclusion, this work showed that nitrocellulose and dacron performed similarly in dot-ELISA, suggesting that they may be used alternatively in population surveillance in endemic areas.
Subject
Infectious Diseases,Microbiology (medical),Parasitology
Reference21 articles.
1. Evaluation of three serological tests for the detectionof human plague in northeast Brazil;Almeida AMP;Memórias do Instituto Oswaldo Cruz,1992
2. Immunoaffinity fractionation of Schistosoma mansoni worm antigens using human antibodies and its application for serodiagnosis;Boctor FN;Immunology,1986
3. Simplification and standardization of dot-ELISA for human schistosomiasis mansoni;Boctor FN;Journal of Parasitology,1987
4. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding;Bradford MM;Analytical Biochemistry,1976
5. Glucose oxidase immobilised on gel beads polyacrylamide and polyethyleneterephthalate;Carvalho Jr LB;Arquivos de Biologia e Tecnologia,1986
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