In vitro maintenance of Angiostrongylus costaricensis does not provide physiological conditions for egg laying

Author:

Mentz Márcia Bohrer1,Graeff-Teixeira Carlos2

Affiliation:

1. Universidade Federal do Rio Grande do Sul, Brasil

2. Pontifícia Universidade Católica de Porto Alegre, Brasil

Abstract

Fifteen pairs (male/female) of Angiostrongylus costaricensis were kept in vitro in Waymouth medium for three days to evaluate the amount and duration of egg laying. At 24, 48 and 72 hours, the mean egg counts were 321, 24 and 4 eggs/10 microliters, respectively. Most of the eggs were eliminated within the first 24 hours, suggesting they are expelled under non-physiological conditions. These results indicate that in vitro conditions are not appropriate for drug trials of egg-laying inhibitors for treatment of abdominal angiostrongylosis.

Publisher

FapUNIFESP (SciELO)

Subject

Infectious Diseases,Microbiology (medical),Parasitology

Reference24 articles.

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2. Angiostrongiloidíase abdominal: seis casos observados no Paraná e em Santa Catarina, Brasil;Ayala MAR;Memórias do Instituto Oswaldo Cruz,1987

3. Angiostrongiloidose abdominal;Ayala MAR;Apresentação de um caso. Memórias do Instituto Oswaldo Cruz,1982

4. Granulomas entéricos e linfáticos com intensa eosinofilia tisular producidos por un strongilídeo (strongylata);Céspedes R;Acta Médica Costaricensis,1967

5. Identificação de roedores silvestres como hospedeiros do Angiostrongylus costaricensis no Sul do Brasil;Graeff-Teixeira C;Revista do Instituto de Medicina Tropical de São Paulo,1990

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