Abstract
AbstractMicroelectrode arrays (MEAs) have applications in drug discovery, toxicology, and basic research. They measure the electrophysiological response of tissue cultures to quantify changes upon exposure to biochemical stimuli. Unfortunately, manual addition of chemicals introduces significant noise in the recordings. Here, we report a simple-to-fabricate fluidic system that addresses this issue. We show that cell cultures can be successfully established in the fluidic compartment under continuous flow conditions and that the addition of chemicals introduces minimal noise in the recordings. This dynamic cell culture system represents an improvement over traditional tissue culture wells used in MEAs, facilitating electrophysiology measurements.
Graphical abstract
Funder
Biotechnology and Biological Sciences Research Council
University of Cambridge Harding Distinguished Postgraduate Scholars Programme
European Union's Horizon 2020 Marie Skłodowska-Curie Innovative Training Network
Yoshida Scholarship Foundation
KDDI Foundation
Shigeta Educational Foundation
EPSRC Cambridge Nanoscience and Nanotechnology (NanoDTC) Ph.D. studentship
Publisher
Springer Science and Business Media LLC