Author:
Wei Haijun,Geng Longwu,Shang Xinchi,Li Lei,Ma Bo,Zhang Yuyong,Li Wang,Xu Wei
Abstract
The Pseudaspius leptocephalus is a unique fish in the Heilongjiang River Basin and has important economic and ecological value. In the present study, the complete mitochondrial genome of P. leptocephalus were determined, and COI partial sequences of 85 individuals from Erguna river (EH), Mohe (MH), Fuyuan (FY), Hulan (HL) were used to evaluated the genetic diversity of four populations of P. leptocephalus in Heilongjiang River Basin. The mitogenome is 16,607 bp in length and contained one D-loop, 2 rRNA, 13 PCG, and 22 tRNA. 4 variable sites and 5 haplotypes were detected in 705 bp COI, and 705 bp COI exhibited a lower content of C + G (45.95%) than A + T (54.05%). The nucleotide diversity (π) and haplotype diversity (h) indices ranged from 0.00027 (HL) to 0.00065 (EH and FY) and from 0.192 (HL) to 0.462 (EH), respectively. The genetic distance within the population and between populations ranged from 0.0006554 to 0.0002728 and from 0.0003541 to 0.0006974, respectively. Pairwise values of FST and Nm showed that there was moderate genetic differentiation between EH population and other populations and individuals between EH population and other populations can mate randomly (0.15 > FST > 0.05, Nm > 4). Significant negative values of neutrality tests (P < 0.05) indicated that MH and FY populations may had experienced population expansion, but mismatch distribution analysis suggested that all populations have remained basically stable. These results provide strong basis for the protection and utilization of P. leptocephalus germplasm resources, and provide valuable information for the population structure and genetic diversity of P. leptocephalus.
Funder
Ministry of Agriculture of the People's Republic of China
Chinese Academy of Fishery Sciences
National Key Research and Development Program of China
Subject
Ocean Engineering,Water Science and Technology,Aquatic Science,Global and Planetary Change,Oceanography
Reference61 articles.
1. Genetic diversity and population structure of selected lacustrine and riverine populations of African catfish, clarias gariepinus (Burchel 1822), in Kenya;Alal;J. Appl. Ichthyol.,2021
2. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing;Bankevich;J. Comput. Biol.,2012
3. Warming-driven shifts in ecological control of fish communities in a large northern Chinese lake over 66 years;Bao;Sci. Total Environ.,2021
4. Phylogenetic analysis of Altai osmans of the genus oreoleuciscus (Pisces, Cyprinidae, Leuciscinae), based on the analysis of the cytochrome oxidase 1 gene (Co-1) sequence;Batishcheva;Genetika,2011
5. MITOS: improved de novo metazoan mitochondrial genome annotation;Bernt;Mol. Phylogenet. Evol.,2013
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