Low-Intensity Pulsed Ultrasound Promotes Osteogenic Potential of iPSC-Derived MSCs but Fails to Simplify the iPSC-EB-MSC Differentiation Process

Abstract

Graphical AbstractWe demonstrate LIPUS at the intensity of 40mW/cm2 and the D.C of 50% (LIPUS40/50) can enhance the osteogenic potential of iMSCs in vitro and in vivo. LIPUS40/50 seems to accelerate EB-iMSC transition, but itfails to convert iPSCs or EB cells into osteoblasts directly. These results represent an important step toward applying iPSCs safely and effectively in bone tissue engineering.