Author:
Li Leijie,Zhang Yujia,Ren Yongyong,Cheng Zhiwei,Zhang Yuening,Wang Xinbo,Zhao Hongyu,Lu Hui
Abstract
Upper gastrointestinal cancer (UGIC) is an aggressive carcinoma with increasing incidence and poor outcomes worldwide. Here, we collected 39,057 cells, and they were annotated into nine cell types. By clustering cancer stem cells (CSCs), we discovered the ubiquitous existence of sub-cluster CSCs in all UGICs, which is named upper gastrointestinal cancer stem cells (UGCSCs). The identification of UGCSC function is coincident with the carcinogen of UGICs. We compared the UGCSC expression profile with 215,291 single cells from six other cancers and discovered that UGCSCs are specific tumor stem cells in UGIC. Exploration of the expression network indicated that inflammatory genes (CXCL8, CXCL3, PIGR, and RNASE1) and Wnt pathway genes (GAST, REG1A, TFF3, and ZG16B) are upregulated in tumor stem cells of UGICs. These results suggest a new mechanism for carcinogenesis in UGIC: mucosa damage and repair caused by poor eating habits lead to chronic inflammation, and the persistent chronic inflammation triggers the Wnt pathway; ultimately, this process induces UGICs. These findings establish the core signal pathway that connects poor eating habits and UGIC. Our system provides deeper insights into UGIC carcinogens and a platform to promote gastrointestinal cancer diagnosis and therapy.
Subject
Biomedical Engineering,Histology,Bioengineering,Biotechnology
Reference68 articles.
1. Areca Nut Chewing and Esophageal Squamous-Cell Carcinoma Risk in Asians: A Meta-Analysis of Case-Control Studies;Akhtar;Cancer Causes Control,2013
2. Glioblastoma: Pathology, Molecular Mechanisms and Markers;Aldape;Acta Neuropathol.,2015
3. Deep Canonical Correlation Analysis;Andrew,2013
4. Gene Ontology: Tool for the Unification of Biology;Ashburner;Nat. Genet.,2000
5. The Gene Ontology Resource: Enriching a GOld Mine;Ashburner;J. Nucl. Acids Res.,2021
Cited by
5 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献