The Influence of Comminuting Methods on the Structure, Morphology, and Calcium Release of Chicken Bones

Abstract

This work aimed at assessing the influence of comminuting methods, including colloid mill, planetary ball mill and dynamic high-pressure microfluidization on the chemical composition, particle properties, morphology and calcium release of chicken bone. The results showed that planetary ball mill and dynamic high-pressure microfluidization could reduce the particle size of bone powder, and the particle size of sample treated by dynamic high-pressure microfluidization reached 446 nm. Chicken bone particles were negatively charged, and the absolute value of zeta potential was significantly reduced after milling treatments. Furthermore, X-ray diffraction and Fourier-transform infrared spectroscopy (FTIR) analysis indicated that the planetary ball mill and dynamic high-pressure microfluidization processes presented no significant effect on the internal chemical structure of bone particles. Compared with the other groups, samples treated by dynamic high-pressure microfluidization released more calcium ions, which was related to the significant effects on surface calcium composition and reducing particle size. Therefore, dynamic high-pressure microfluidization has a great potential in the processing of bone-derived products, particularly for the design and development of bone-derived product with high calcium bioaccessibility.