Author:
Liu Xiaofeng,Zeeshan Ul Haq Muhammad,Yu Jing,Liu Ya,Yang Huageng,Cui Hongguang,Yang Dongmei,Wu Yougen
Abstract
To further reveal the molecular mechanisms underlying the formation of continuous cropping disorders in patchouli, this study analyzed the function of calcium dependent protein kinase (CDPK) genes at the molecular level in patchouli continuous cropping disorders. The findings unveiled the presence of 45 PcCDPK genes within the complete patchouli genome dataset. These genes exhibited a range of molecular weights from 50.78 to 78.96 kDa and aliphatic index values spanning from 74.42 to 88.49, and are shown to be hydrophilic proteins. The evolution of 45 PcCDPK members was divided into 4 subfamilies, with a total of 65 pairs of collinear genes. Each PcCDPK contains a STKc-CAMK domain and four EF-hand structures exhibiting a certain degree of conservatism during evolution. Transcriptome data further supported the significance of PcCDPK25 and PcCDPK38 genes, showing substantial upregulation, which was corroborated by qRT-PCR results. The 1629 bp and 1716 bp CDS sequences were obtained by cloning the PcCDPK25 and PcCDPK38 genes, respectively, and subcellular localization showed that both proteins were localized on the plasma membrane. This comprehensive study provides molecular-level confirmation of the pivotal roles played by CDPK genes in the emergence of continuous cropping challenges in patchouli plants, establishing a crucial foundation for a deeper comprehension of the molecular mechanisms underpinning these obstacles.
Funder
National Natural Science Foundation of China
Key Research and Development Project of Hainan Province
Reference62 articles.
1. A rice calcium-dependent protein kinase OsCPK12 oppositely modulates salt-stress tolerance and blast disease resistance;Asano;Plant J.,2012
2. CDPK-mediated abiotic stress signaling;Asano;Plant Signal. Behav.,2012
3. The MEME suite;Bailey;Nucleic Acids Res.,2015
4. Cycloheximide induces a subset of low temperature-inducible genes in maize;Berberich;Mol. Gen. Genet.,1997
5. Autotoxicity of phenolic compounds from the soil of American ginseng (Panax quinquefolium L.);Bi;Allelopathy J.,2010
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