A Photosynthetic Light Acclimation Model Accounting for the Effects of Leaf Age, Chlorophyll Content, and Intra-Leaf Radiation Transfer

Abstract

Mechanistic models of canopy photosynthesis usually upscale leaf photosynthesis to crop level. A detailed prediction of canopy microclimate with accurate leaf morphological and physiological model parameters is the pre-requisite for accurate predictions. It is well established that certain leaf model parameters (Vcmax, Jmax) of the frequently adopted Farquhar and Caemmerer photosynthesis model change with leaf age and light interception history. Previous approaches to predict Vcmax and Jmax focused primarily on light interception, either by cumulative intercepted photosynthetic photon flux density (PPFD) or by closely related proxy variables such as leaf nitrogen content per leaf area. However, for plants with monopodial growth, such as vertically grown tomatoes or cucumber crops, in greenhouse production, there is a strong relationship between leaf age and light interception, complicating the experimental and mathematical separation of both effects. We propose a modeling framework that separates age and light intensity-related acclimation effects in a crop stand: Improved approximation of intra-leaf light absorption profiles with cumulative chlorophyll content (Chl) is the basis, while parameters are estimated via Gaussian process regression from total Chl, carotenoid content (Car), and leaf mass per area (LMA). The model approximates light absorption profiles within a leaf and links them to leaf capacity profiles of photosynthetic electron transport. Published datasets for Spinacia oleracea and Eucalyptus pauciflora were used to parameterize the relationship between light and capacity profiles and to set the curvature parameter of electron transport rate described by a non-rectangular hyperbola on Cucumis sativus. Using the modified capacity and light absorption profile functions, the new model was then able to predict light acclimation in a 2-month period of a fully grown tomato crop. An age-dependent lower limit of the electron transport capacity per unit Chl was essential in order to capture the decline of Vcmax and Jmax over time and space of the investigated tomato crop. We detected that current leaf photosynthetic capacity in tomato is highly affected by intercepted light-sum of 3–5 previous days.