Author:
Almagro Jorge,Messal Hendrik A.
Abstract
Volume imaging visualizes the three-dimensional (3D) complexity of tumors to unravel the dynamic crosstalk between cancer cells and the heterogeneous landscape of the tumor microenvironment (TME). Tissue clearing and intravital microscopy (IVM) constitute rapidly progressing technologies to study the architectural context of such interactions. Tissue clearing enables high-resolution imaging of large samples, allowing for the characterization of entire tumors and even organs and organisms with tumors. With IVM, the dynamic engagement between cancer cells and the TME can be visualized in 3D over time, allowing for acquisition of 4D data. Together, tissue clearing and IVM have been critical in the examination of cancer-TME interactions and have drastically advanced our knowledge in fundamental cancer research and clinical oncology. This review provides an overview of the current technical repertoire of fluorescence volume imaging technologies to study cancer and the TME, and discusses how their recent applications have been utilized to advance our fundamental understanding of tumor architecture, stromal and immune infiltration, vascularization and innervation, and to explore avenues for immunotherapy and optimized chemotherapy delivery.
Funder
Cancer Research UK
KWF Kankerbestrijding
Nederlandse Organisatie voor Wetenschappelijk Onderzoek
Subject
Immunology,Immunology and Allergy
Reference187 articles.
1. Pembrolizumab for the treatment of non-small-cell lung cancer;Garon;N Engl J Med,2015
2. Multiplexed 3D atlas of state transitions and immune interaction in colorectal cancer;Lin;Cell,2023
3. Coordinated cancer chaos;Messal;Cell,2023
4. Über das durchsichtigmachen von menschlichen und tierischen präparaten und seine theoretischen bedingungen;Spalteholz;S. Hirzel Leipzig,1914
5. Tissue clearing to examine tumour complexity in three dimensions;Almagro;Nat Rev Cancer,2021
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