Complete genome sequence, metabolic model construction, and huangjiu application of Saccharopolyspora rosea A22, a thermophilic, high amylase and glucoamylase actinomycetes

Abstract

Saccharopolysporais an important microorganism in the fermentation process of wheatquandhuangjiu, yet the mechanisms by which it performs specific functions inhuangjiuremain unclear. A strain with high amylase and glucoamylase activities was isolated from wheatquand identified asSaccharopolyspora rosea(S. rosea) A22. We initially reported the whole genome sequence ofS. roseaA22, which comprised a circular chromosome 6,562,638 bp in size with a GC content of 71.71%, and 6,118 protein-coding genes. A functional genomic analysis highlighted regulatory genes involved in adaptive mechanisms to harsh conditions, andin vitroexperiments revealed that the growth ofS. roseaA22 could be regulated in response to the stress condition. Based on whole-genome sequencing, the first genome-scale metabolic model ofS. roseaA22 namediSR1310 was constructed to predict the growth ability on different media with 91% accuracy. Finally,S. roseaA22 was applied tohuangjiufermentation by inoculating raw wheatqu, and the results showed that the total higher alcohol content was reduced by 12.64% compared with the control group. This study has elucidated the tolerance mechanisms and enzyme-producing properties ofS. roseaA22 at the genetic level, providing new insights into its application tohuangjiu.