EFFECTS OF SELECTION IN TERMS OF MEAT YIELD TRAITS ON LEPTIN RECEPTOR GENE IN JAPANESE QUAIL LINES

Abstract

Abstract: This study was carried out to investigate the effects of selection on the single nucleotide polymorphisms (SNPs) in coding sequence of leptin receptor (LEPR) gene and possible associations between SNPs’ and some meat yield traits of Japanese quail lines. Fifteen generations divergently selected two lines (HBW and LBW) for 5-weeks of age body weight and a control were used as materials for this study. A 348-bp part of the LEPR coding region (18th exon) were sequenced in a total of 113 individuals from the three quail lines and shown that the fragments contained four SNPs loci (T490C, C528T, G537A, T571C) and five haplotypes (TTGT, CTGT, TCGT, TCAT, TCAC). T490C replacement caused the missense mutation of phenylalanine to convert to leucine (PheLeu). However, other SNPs were synonymous and there were no changes in transcripts. It was determined that the quails with higher phenotypic values were in the TT genotype at the T390C locus. Statistical analyses showed that there were significant differences among the quail lines, SNP alleles and haplotypes in terms of interested phenotypic traits (P0.05), and also SNP and haplotype distributions changed depending on quail lines (P0.001). When all results were evaluated together, it was concluded that the fifteen generations of selection caused significant changes in the LEPR gene in terms of economically important traits in Japanese quail lines (C. coturnix japonica). Key words: SNP; haplotype; leptin receptor; selection; Japanese quail0.05) and also SNP and haplotype distributions changed depending on quail lines (P0.001). When all results were evaluated together, it was concluded that the fifteen generations of selection caused significant changes in the LEPR gene in terms of economically important traits in Japanese quail lines (C. coturnix japonica).Key words: SNP; haplotype; leptin receptor; selection; Japanese quailUČINKI SELEKCIJE LINIJ JAPONSKIH PREPELIC NA GEN ZA LEPTINSKI RECEPTOR, POVEZAN S PRIREJO MESAIzvleček: Ta študija je bila izvedena z namenom raziskati učinke selekcije na polimorfizme posameznih nukleotidov (Angl., Single Nucleotide Polymorphism, SNP) v kodnem zaporedju gena za leptinski receptor (LEPR) in možne povezave med SNP in nekaterimi značilnostmi prireje mesa pri japonskih prepelicah. V študiji je bilo poleg kontrole uporabljenih petnajst generacij različno izbranih dveh linij (HBW in LBW) s telesno maso pri starosti petih tednov. 348-bp del kodne regije LEPR (18. ekson) je bil sekvenciran pri skupno 113 posameznikih iz treh linij prepelic, fragmenti pa so vsebovali štiri lokuse SNP (T490C, C528T, G537A, T571C) in pet haplotipov (TTGT, CTGT, TCGT, TCAT, TCAC). Zamenjava T490C je povzročila drugačnosmiselno mutacijo fenilalanina v levcin (Phe Leu), vendar so bili drugi SNP-ji sinonimni in v transkriptih ni bilo sprememb. Ugotovljeno je bilo, da so prepelice z višjimi fenotipskimi vrednostmi imele genotip TT na lokusu T390C. Statistične analize so z vidika fenotipskih lastnosti pokazale značilne razlike med linijami prepelic, aleli SNP in haplotipi (P 0,05), med linijami prepelic pa je bila različna tudi porazdelitev SNP in haplotipov (P 0,001). Na podlagi vrednotenja vseh rezultatov smo ugotovili, da je selekcija petnajstih generacij linij japonskih prepelic (C. coturnix japonica) povzročila ključne spremembe v genu LEPR, povezanim z gospodarsko pomembnimi lastnostmi prepelic.Ključne besede: SNP; haplotip; leptinski receptor; selekcija; japosnka prepelica