Prevalence and Molecular Characterization of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in Cattle in Heilongjiang Province, Northeast China
Author:
Gao Jun-Feng1ORCID, Zhou Lu1, Zhang Ai-Hui1, Hou Mei-Ru1, Liu Xue-Wei1, Zhang Xin-Hui1, Wang Jia-Wen1, Wang Xue1, Bai Xue1, Jiao Chen-Long1, Yang Yan1, Lan Zhuo1, Qiu Hong-Yu1ORCID, Wang Chun-Ren1ORCID
Affiliation:
1. Key Laboratory of Bovine Disease Control in Northeast China, Ministry of Agriculture and Rural Affair, Key Laboratory of Prevention and Control of Zoonotic Diseases of Daqing, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China
Abstract
Crytosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi are important diarrheal pathogens with a global distribution that threatens the health of humans and animals. Despite cattle being potential transmission hosts of these protozoans, the associated risks to public health have been neglected. In the present study, a total of 1155 cattle fecal samples were collected from 13 administrative regions of Heilongjiang Province. The prevalence of Cryptosporidium spp., G. duodenalis, and E. bieneusi were 5.5% (64/1155; 95% CI: 4.2–6.9), 3.8% (44/1155; 95% CI: 2.7–4.9), and 6.5% (75/1155; 95% CI: 5.1–7.9), respectively. Among these positive fecal samples, five Cryptosporidium species (C. andersoni, C. bovis, C. ryanae, C. parvum, and C. occultus), two G. duodenalis assemblages (E and A), and eight E. bieneusi genotypes (BEB4, BEB6, BEB8, J, I, CHS7, CHS8, and COS-I) were identified. Phylogenetic analysis showed that all eight genotypes of E. bieneusi identified in the present study belonged to group 2. It is worth noting that some species/genotypes of these intestinal protozoans are zoonotic, suggesting a risk of zoonotic disease transmission in endemic areas. The findings expanded our understanding of the genetic composition and zoonotic potential of Cryptosporidium spp., G. duodenalis, and E. bieneusi in cattle in Heilongjiang Province.
Funder
National Natural Science Foundation of China Heilongjiang Provincial Natural Science Foundation of China Heilongjiang Bayi Agricultural University support Program for San Heng San Zong Personnel Foundation of Heilongjiang Bayi Agricultural University
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