Affiliation:
1. College of Food Science, South China Agricultural University, Guangzhou 510642, China
2. Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China
Abstract
Intestinal diseases caused by sleep deprivation (SD) are severe public health threats worldwide. However, whether or not probiotics attenuate the intestinal damage associated with SD remains unclear. In this study, we used antibiotic pretreatment and fecal microbiota transplantation to investigate the protective role of Lactiplantibacillus plantarum (L. plantarum) 124 against SD-related intestinal barrier damage in C57BL/6 mice. Compared with those of a normal sleeping mouse, we observed that intestinal antioxidant capacity and anti-inflammatory cytokine levels were decreased, while pro-inflammatory cytokines were increased in sleep deprivation mice with an increasing duration of sleep deprivation. This resulted in decreased tight junction protein expression and increased intestinal barrier permeability. In contrast, intragastric administration with L. plantarum 124 reversed SD-associated intestinal oxidative stress, inflammation, colonic barrier damage, and the dysbiosis of the microbiota in the colon. In addition, L. plantarum 124 restored gut microbiota homeostasis via restoring abundance, including that of Dubosiella, Faecalibaculum, Bacillus, Lachnoclostridium, and Bifidobacterium. Further studies showed that gut microbiota mediated SD-associated intestinal damage and the treatment L. plantarum 124 in SD-associated colonic barrier damage. L. plantarum 124 is a potential candidate for alleviating SD-associated intestinal barrier damage. Overall, L. plantarum 124 consumption attenuates intestinal oxidative stress, inflammation, and intestinal barrier damage in SD-associated mice via the modulation of gut microbes.
Funder
Key Research and Development Program of Guangdong Province
Department of Science and Technology of Guangdong Province
GDAS’ Project of Science and Technology Development
Subject
Food Science,Nutrition and Dietetics
Reference41 articles.
1. Sleep problems during the COVID-19 pandemic by population: A systematic review and meta-analysis;Jahrami;J. Clin. Sleep Med.,2021
2. Sleep disturbances during the COVID-19 pandemic: A systematic review, meta-analysis, and meta-regression;Jahrami;Sleep Med. Rev.,2022
3. Gender-specific estimates of sleep problems during the COVID-19 pandemic: Systematic review and meta-analysis;Alimoradi;J. Sleep Res.,2022
4. Relationship between sleep disorders and gut dysbiosis: What affects what?;Neroni;Sleep Med.,2021
5. Altevogt, B.M., and Colten, H.R. (2006). Sleep Disorders and Sleep Deprivation: An Unmet Public Health Problem, National Academies Press.