Characterisation of New Foxunavirus Phage Murka with the Potential of Xanthomonas campestris pv. campestris Control-Reference-Cited by-同舟云学术

Characterisation of New Foxunavirus Phage Murka with the Potential of Xanthomonas campestris pv. campestris Control

Published:2024-01-27 Issue:2 Volume:16 Page:198
ISSN:1999-4915
Container-title:Viruses
language:en
Short-container-title:Viruses

Author:

Evseev Peter V.¹²³^ORCID,Tarakanov Rashit I.¹^ORCID,Vo Ha T. N.⁴^ORCID,Suzina Natalia E.⁵^ORCID,Vasilyeva Anna A.¹,Ignatov Alexander N.¹⁶^ORCID,Miroshnikov Konstantin A.¹²^ORCID,Dzhalilov Fevzi S.-U.¹^ORCID

Affiliation:

1. Department of Plant Protection, Russian State Agrarian University—Moscow Timiryazev Agricultural Academy, Timiryazevskaya Str. 49, 127434 Moscow, Russia

2. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str. 16/10, 117997 Moscow, Russia

3. Laboratory of Molecular Microbiology, Pirogov Russian National Research Medical University, Ostrovityanova 1, 117997 Moscow, Russia

4. Faculty of Agronomy, Nong Lam University, Quarter 6, Thu Duc District, Ho Chi Minh City 721400, Vietnam

5. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Center for Biological Research of the Russian Academy of Sciences”, Prosp. Nauki, 5, 142290 Pushchino, Russia

6. Agrobiotechnology Department, Agrarian and Technological Institute, RUDN University, Miklukho-Maklaya Str., 6, 117198 Moscow, Russia

Abstract

Phages of phytopathogenic bacteria are considered to be promising agents for the biological control of bacterial diseases in plants. This paper reports on the isolation and characterisation of a new Xanthomonas campestris pv. campestris phage, Murka. Phage morphology and basic kinetic characteristics of the infection were determined, and a phylogenomic analysis was performed. The phage was able to lyse a reasonably broad range (64%, 9 of the 14 of the Xanthomonas campestris pv. campestris strains used in the study) of circulating strains of the cabbage black rot pathogen. This lytic myovirus has a DNA genome of 44,044 bp and contains 83 predicted genes. Taxonomically, it belongs to the genus Foxunavirus. This bacteriophage is promising for use as a possible means of biological control of cabbage black rot.

Funder

Ministry of Science and Higher Education of the Russian Federation

“Future Agrotechnologies” scientific centre

Publisher

MDPI AG

Link

https://www.mdpi.com/1999-4915/16/2/198/pdf

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