An ELISA to Detect Antibodies to Bovine Alphaherpesviruses 1 and 5 and Bubaline Alphaherpesvirus 1 in Cattle Sera
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Published:2023-02-02
Issue:2
Volume:10
Page:110
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ISSN:2306-7381
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Container-title:Veterinary Sciences
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language:en
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Short-container-title:Veterinary Sciences
Author:
Scheffer Camila Mengue12, Petzhold Sylio Alfredo1, Varela Ana Paula Muterle12, Paim Willian Pinto12, Duarte Phelipe Magalhães3, Loiko Márcia Regina12, Cerva Cristine1, Schmidt Candice12, Wendlant Adrieli12, Cibulski Samuel Paulo12ORCID, Lima Diane Alves de12, Tochetto Caroline12, Santos Anne Caroline Ramos dos12, Herpich Juliana Inês12, Teixeira Thais Fumaco2, Santos Helton Fernandes dos2, Campos Fabrício Souza4ORCID, Franco Ana Cláudia14ORCID, Roehe Paulo Michel14
Affiliation:
1. Programa de Pós-Graduação em Ciências Veterinárias, Faculdade de Medicina Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre 90650-002, Brazil 2. Centro de Pesquisa em Saúde Animal, Instituto de Pesquisas Veterinárias Desidério Finamor, Departamento de Diagnóstico e Pesquisa Agropecuária, Secretaria de Agricultura, Pecuária e Desenvolvimento Rural, Eldorado do Sul 90150-004, Brazil 3. Curso de Biomedicina, Campus Primavera do Leste, Universidade de Cuiabá, Cuiabá 78065-900, Brazil 4. Laboratório de Virologia, Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90035-003, Brazil
Abstract
Bovine alphaherpesvirus 1 (subtypes 1.1, 1.2a, and 1.2b), type 5 (subtypes 5a, 5b, and 5c), and bubaline herpesvirus 1 (BuHV-1) induce highly, though not fully cross-reactive serological responses. Most types and subtypes of these viruses circulate particularly in countries of the southern hemisphere, notably Brazil and Argentina. Therefore, the detection of infected animals is important in defining prevention and control strategies, particularly when flocks are destined for international trade. Identification of infected herds is most often achieved by assays that detect antibodies, such as enzyme immunoassays (ELISAs). However, to date, no ELISA has been evaluated in its capacity to detect antibodies to these alphaherpesviruses. Here, an ELISA was developed to detect antibodies to all currently recognized BoAHV-1, BoAHV-5, and BuAHV-1 types/subtypes, and its sensitivity and specificity were determined. Six hundred bovine sera were screened in serum neutralization tests (SN) against the seven viruses. ELISAs prepared with each of the viruses were compared to SN. Subsequently, a combined assay with multiple antigens LISA was prepared by mixing five viral antigens, chosen for their highest sensitivity in the preparative assays. In comparison to SN, the mAgELISA sensitivity was 96.5% with 96.1% specificity (κ = 0.93; PPV = 95.0%; NPV = 97.3%). The findings reveal that the mAgELISA developed here is highly suitable for the detection of antibodies, comparable in sensitivity and specificity to that of SN when performed with all known types and subtypes of bovine and bubaline alphaherpesviruses.
Funder
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico Financiadora de Estudos e Projetos FAPERGS
Subject
General Veterinary
Reference35 articles.
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