Affiliation:
1. Department of Mechanical Engineering, University of Akron, Akron, OH 44325, USA
2. Department of Biomedical Engineering, University of Akron, Akron, OH 44325, USA
Abstract
The fast, accurate detection of biomolecules, ranging from nucleic acids and small molecules to proteins and cellular secretions, plays an essential role in various biomedical applications. These include disease diagnostics and prognostics, environmental monitoring, public health, and food safety. Aptamer recognition (DNA or RNA) has gained extensive attention for biomolecular detection due to its high selectivity, affinity, reproducibility, and robustness. Concurrently, biosensing with nanoparticles has been widely used for its high carrier capacity, stability and feasibility of incorporating optical and catalytic activity, and enhanced diffusivity. Biosensors based on aptamers and nanoparticles utilize the combination of their advantages and have become a promising technology for detecting of a wide variety of biomolecules with high sensitivity, reliability, specificity, and detection speed. Via various sensing mechanisms, target biomolecules have been quantified in terms of optical (e.g., colorimetric and fluorometric), magnetic, and electrical signals. In this review, we summarize the recent advances in and compare different aptamer–nanoparticle-based biosensors by nanoparticle types and detection mechanisms. We also share our views on the highlights and challenges of the different nanoparticle-aptamer-based biosensors.
Funder
National Science Foundation of USA
National Institute of Health of USA
Subject
Clinical Biochemistry,General Medicine,Analytical Chemistry,Biotechnology,Instrumentation,Biomedical Engineering,Engineering (miscellaneous)
Reference133 articles.
1. Structural motifs of biomolecules;Banavar;Proc. Natl. Acad. Sci. USA,2007
2. Essential Biomolecules in Food Webs;Ruess;Front. Ecol. Evol.,2019
3. In Vivo Profiling and Visualization of Cellular Protein-Lipid Interactions Using Bifunctional Fatty Acids;Haberkant;Angew. Chem. Int. Ed.,2013
4. SERS-based detection of biomolecules;Cialla;Nanophotonics,2014
5. Huang, Y., Xu, J., Liu, J., Wang, X., and Chen, B. (2017). Disease-Related Detection with Electrochemical Biosensors: A Review. Sensors, 17.
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