Affiliation:
1. State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
2. Sanya Institute of China Agricultural University, Sanya 572000, China
Abstract
To investigate the effect of phenyllactic acid (PLA) on the dynamic changes of high-moisture stylo silage, fresh stylo was ensiled with addition of PLA at the levels of 0, 1% and 2% using lab-level silage bags, where samples were collected on days 3, 7, 14 and 30 of ensiling fermentation to analyze fermentation parameters, nitrogen distribution and bacterial community. The results showed that PLA addition at ensiling led to the increase (p < 0.01) in dry matter content, lactic acid concentration and Flieg’s score of stylo silage as well as the decrease (p < 0.01) in dry matter loss, pH value and coliform bacteria population, with butyric acid only detected in the control group. It also resulted in the increase (p < 0.01) in true protein content and its proportion as well as the decrease (p < 0.01) in ammonia-N content and its proportion, almost with linearly dose effect. Sequencing analysis revealed that PLA addition led to the increase (p < 0.05) in Sobs, Shannon, Chao and Ace of bacterial community in the stylo silage, where the relative abundance of Enterobacter, Clostridium, and Kosakonia was decreased, and that of Lactobacillus, Enterococcus and Pantoea was increased. Furthermore, Kosakonia, Terriglobus, Sphingomonas and Sphingopyxis had an important role in the bacterial interaction network. It is suggested that PLA application at the level of 1–2% could improve silage quality of stylo silage via modifying bacterial community.
Funder
Hainan Provincial Natural Science Foundation of China
Agricultural Research System of MOF and MARA
National Natural Science Foundation of China
Subject
Plant Science,Biochemistry, Genetics and Molecular Biology (miscellaneous),Food Science
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