Promising Insecticidal Properties of Essential Oils from Artemisia aragonensis Lam. and Artemisia negrei L. (Asteraceae) by Targeting Gamma-Aminobutyric Acid and Ryanodine Receptor Proteins: In Vitro and In Silico Approaches

Author:

Chebbac Khalid1ORCID,Benziane Ouaritini Zineb2,Allali Aimad3ORCID,Tüzün Burak4ORCID,Zouirech Otmane5,Chalkha Mohammed6ORCID,El Moussaoui Abdelfattah7ORCID,Lafraxo Soufyane7,Nafidi Hiba-Allah8,Bin Jardan Yousef A.9ORCID,Bourhia Mohammed10,Guemmouh Raja1

Affiliation:

1. Laboratory of Biotechnology Conservation and Valorisation of Natural Resources, Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdallah University, Fez 30000, Morocco

2. Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health and Quality of Life, Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez 30000, Morocco

3. Laboratory of Plant Animal and Agro-Industry Productions, Faculty of Sciences, University of Ibn Tofail, Kenitra 14000, Morocco

4. Department of Plant and Animal Production, Technical Sciences Vocational School of Sivas, Sivas Cumhuriyet University, Sivas 58140, Turkey

5. Laboratory of Natural Substances, Pharmacology Environment, Modeling, Health and Quality of Life, Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdellah University, Fez 30000, Morocco

6. Engineering Laboratory of Organometallic, Molecular, Materials and Environment, Faculty of Sciences Dhar EL Mahraz, Sidi Mohamed Ben Abdellah University, 30000 Fez, Morocco

7. Laboratory of Biotechnology, Environment, Agri-Food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdellah University, Fez 30000, Morocco

8. Department of Food Science, Faculty of Agricultural and Food Sciences, Laval University, 2325 Rue de l’Université, Quebec City, QC G1V 0A6, Canada

9. Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia

10. Laboratory of Chemistry and Biochemistry, Faculty of Medicine and Pharmacy, Ibn Zohr University, Laayoune 70000, Morocco

Abstract

Artemisia negrei (A. negrei) and Artemisia aragonensis (A. aragonensis) are in the family Asteraceae, which has been used in traditional medicine. The use of plant-derived insecticides has become a promising strategy to reduce the harmful effects of synthetic insecticides and overcome the bio-resistance of pest insects to insecticides. In this regard, the purpose of the current study was to determine the chemical composition and evaluate insecticidal effects of essential oils (EOs) extracted from A. negrei (EON) and A. aragonensis (EOA). Notably, all chemical constituents present in the EOs were identified through GC-MS analysis, whilst the insecticidal properties against Callosobruchus maculatus Fab. (C. maculatus) were investigated by use of in vitro an in silico approaches. The obtained results showed that both tested EOs present a significant insecticidal effect against C. maculatus, which increased significantly upon the dose used in both contact and inhalation tests. The lethal concentrations (LC50) for the inhalation test were found to be 2.1 and 2.97 μL/L, while in the contact test they were 2.08 and 2.74 μL/L of air for EON and EOA, respectively. At 5 μL/L of air, the spawn reduction rate was 88.53 % and 77.41%, while the emergence reduction rate was 94.86% and 81.22% by EON and EOA, respectively. With increasing doses of up to 20 μL/L of air, the reduction in individual emergence reached 100% by the two oils tested after 36 h of treatment. In addition, Molecular docking (MD) simulations supported the in vitro findings and indicated that certain identified components in EOA and EON exhibited stronger hydrogen bonding interactions with the target receptors. Interestingly, the prediction of ADMET properties indicates that the molecules investigated have great pharmacokinetic profiles with no side effects. Taken together, our findings suggest that EOA and EON may exert both potential contact and inhalation insecticidal actions and could be used as an alternative tool for the control of this major insect pest of stored products.

Funder

Researchers Supporting Project

King Saud University

Publisher

MDPI AG

Subject

Filtration and Separation,Analytical Chemistry

Reference76 articles.

1. Musolin, D.L., Kirichenko, N.I., Karpun, N.N., Aksenenko, E.V., Golub, V.B., Kerchev, I.A., Mandelshtam, M.Y., Vasaitis, R., Volkovitsh, M.G., and Zhuravleva, E.N. (2022). Invasive insect pests of forests and urban trees in Russia: Origin, pathways, damage, and management. Forests, 13.

2. Regulating agricultural intensification: Lessons from West Africa’s rapidly growing pesticide markets;Haggblade;Dev. Policy Rev.,2022

3. MMPI-2 for Mexico: Translation and adaptation;GM;J. Pers. Assess.,1994

4. Elabora IPN frijol instantáneo altamente nutritivo;Nota Periodís. El Univers. Ed.,2007

5. Aceites esenciales para controlar Acanthoscelides obtectus (Say) y Sitophilus zeamais (Motschulsky) plagas de granos almacenados;Rev. Mex. Agroecosistemas,2016

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3