Abstract
Lyme borreliosis is a vector-borne disease in humans and animals caused by bacteria from the Borrelia burgdorferi sensu lato complex (Bbsl). The possible transmission of Bbsl from companion animals to humans via ticks makes this disease important in terms of One Health approaches. Thus, early and accurate diagnosis and treatment are of utmost importance. Today’s standard for the detection of specific antibodies against Bbsl is a two-tiered test system based on an ELISA for screening combined with a line immunoassay (LIA) for confirmation. In this study, 200 canine and 200 equine serum samples with known antibody status were tested with two different LIAs (A and B). Results were compared regarding sensitivity, specificity, the diagnostic outcome for dogs and horses, as well as operability of the test. The results for canine serum samples corresponded to 94.0%, making both LIAs a good choice for LB diagnostic in dogs. For equine serum samples, the agreement of both tests was 65.5%, displaying the challenge equine samples still provide in LB diagnostic. Major concerns were the interpretation of the OspA antigen (AG) signal and the use of unspecific (i.e., p100/p83) or too sensitive signals on the LIA. The operability of both LIAs was equally user-friendly. Regarding the tests’ evaluation, the scanning process provided by LIA A was a major advantage considering the comparability of the tests.
Reference68 articles.
1. Prevalence and risk factors for selected canine vector-borne diseases in Greece;Angelou;Parasites Vectors,2019
2. Prevalence of antibodies against Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum and their clinical relevance in dogs in Munich, Germany;Barth;Berl. Münchener Tierärztliche Wochenschr.,2012
3. Gerber, B., Eichenberger, S., Wittenbrink, M.M., and Reusch, C.E. (2007). Increased prevalence of Borrelia burgdorferi infections in Bernese Mountain Dogs: A possible breed predisposition. BMC Vet. Res., 3.
4. Seroprevalence of Borrelia burgdorferi in horses presented for coggins testing in Southwest Virginia and change in positive test results approximately 1 year later;Funk;J. Vet. Intern. Med.,2016
5. Dogs and horses with antibodies to outer-surface protein C as on-time sentinels for ticks infected with Borrelia burgdorferi in New York State in 2011;Wagner;Prev. Vet. Med.,2012