The Effect of Foam-Recycle on Ammonium Removal by Aerobic Denitrification Using Alcaligenes faecalis No. 4

Abstract

Aerobic denitrifier Alcaligenes faecalis No. 4 removes ammonium-nitrogen to nitrogen gas via denitrification in a single aerobic condition. In our previous studies, factors such as ammonium removal rate, denitrification ratio, and cell growth were tested in various conditions. The removal pathway from ammonium to nitrogen gas still needs to be determined in detail. To clarify this pathway of Alcaligenes faecalis No. 4, we in this study investigated the effects of several factors on ammonium removal, such as foam-recycle, initial pH, initial ammonium concentration, and airflow rate. Denitrification ratio was improved by up to 23% through foam-recycle. The improvement of the denitrification ratio was resulted by the higher enzyme activity of hydroxylamine oxidoreductase (HAO) in the produced foam, which was about 28 times higher than that in the culture broth (i.e., without foam-recycle). The stripped ammonia was significantly high (above pH 9). The initial ammonium concentration and airflow rate also influenced the denitrification ratio.