Characterization and Biological Activities of In Vitro Digested Olive Pomace Polyphenols Evaluated on Ex Vivo Human Immune Blood Cells
-
Published:2023-02-24
Issue:5
Volume:28
Page:2122
-
ISSN:1420-3049
-
Container-title:Molecules
-
language:en
-
Short-container-title:Molecules
Author:
Alimenti Claudio1, Lianza Mariacaterina2ORCID, Antognoni Fabiana2ORCID, Giusti Laura3ORCID, Bistoni Onelia4, Liotta Luigi5ORCID, Angeloni Cristina2ORCID, Lupidi Giulio3, Beghelli Daniela1ORCID
Affiliation:
1. School of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino, Italy 2. Department for Life Quality Studies, Alma Mater Studiorum, University of Bologna, 47921 Rimini, Italy 3. School of Pharmacy, University of Camerino, 62032 Camerino, Italy 4. Rheumatology Unit, Department of Medicine, University of Perugia, 06126 Perugia, Italy 5. Department of Veterinary Science, University of Messina, 98168 Messina, Italy
Abstract
Olive pomace (OP) represents one of the main by-products of olive oil production, which still contains high quantities of health-promoting bioactive compounds. In the present study, three batches of sun-dried OP were characterized for their profile in phenolic compounds (by HPLC-DAD) and in vitro antioxidant properties (ABTS, FRAP and DPPH assays) before (methanolic extracts) and after (aqueous extracts) their simulated in vitro digestion and dialysis. Phenolic profiles, and, accordingly, the antioxidant activities, showed significant differences among the three OP batches, and most compounds showed good bioaccessibility after simulated digestion. Based on these preliminary screenings, the best OP aqueous extract (OP-W) was further characterized for its peptide composition and subdivided into seven fractions (OP-F). The most promising OP-F (characterized for its metabolome) and OP-W samples were then assessed for their potential anti-inflammatory properties in ex vivo human peripheral mononuclear cells (PBMCs) triggered or not with lipopolysaccharide (LPS). The levels of 16 pro-and anti-inflammatory cytokines were measured in PBMC culture media by multiplex ELISA assay, whereas the gene expressions of interleukin-6 (IL-6), IL-10 and TNF-α were measured by real time RT-qPCR. Interestingly, OP-W and PO-F samples had a similar effect in reducing the expressions of IL-6 and TNF-α, but only OP-W was able to reduce the release of these inflammatory mediators, suggesting that the anti-inflammatory activity of OP-W is different from that of OP-F.
Subject
Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science
Reference72 articles.
1. Kaza, S., Yao, L., Bhada-Tata, P., Van Woerden, F., Lonkova, K., Morton, J., Poveda, R.A., Sarraf, M., Malkawi, F., and Harinath, A.S. (2018). What a Waste 2.0: A Global Snapshot of Solid Waste Management to 2050, World Bank Group. 2. Tapia-Quirós, P., Montenegro-Landívar, M.F., Reig, M., Vecino, X., Cortina, J.L., Saurina, J., and Granados, M. (2022). Recovery of Polyphenols from Agri-Food By-Products: The Olive Oil and Winery Industries Cases. Foods, 11. 3. New Advances in the Integrated Management of Food Processing By-Products in Europe: Sustainable Exploitation of Fruit and Cereal Processing by-Products with the Production of New Food Products (NAMASTE EU);Fava;New Biotechnol.,2013 4. Potential Use of Plant-based By-products and Waste to Improve the Quality of Gluten-free Foods;Difonzo;J. Sci. Food Agric.,2022 5. Malekjani, N., and Jafari, S.M. (2022). Valorization of Olive Processing By-Products via Drying Technologies: A Case Study on the Recovery of Bioactive Phenolic Compounds from Olive Leaves, Pomace, and Wastewater. Crit. Rev. Food Sci. Nutr., 1–19.
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|