Abstract
The study of the molecular mechanisms of the pathogenesis of Alzheimer’s disease (AD) is extremely important for identifying potential therapeutic targets as well as early markers. In this regard, the study of the role of post-translational modifications (PTMs) of β-amyloid (Aβ) peptides is of particular relevance. Serine-8 phosphorylated forms (pSer8-Aβ) have been shown to have an increased aggregation capacity and may reflect the severity of amyloidosis. Here, an approach for quantitative assessment of pSer8-Aβ based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is proposed. The relative fraction of pSer8-Aβ was estimated in the total Aβ-pool with a detection limit of 1 fmol for pSer8-Aβ (1–16) and an accuracy of 2% for measurements in the reflectron mode. The sensitivity of the developed method is suitable for determining the proportion of phosphorylated peptides in biological samples.
Funder
Ministry of Science and Higher Education of the Russian Federation
Subject
Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science
Reference18 articles.
1. 2021 Alzheimer’s Disease Facts and Figures;Gaugler;Alzheimer’s Dement.,2021
2. The Global Prevalence of Dementia: A Systematic Review and Metaanalysis;Prince;Alzheimer’s Dement.,2013
3. Amyloid Beta: Structure, Biology and Structure-Based Therapeutic Development;Chen;Acta Pharmacol. Sin.,2017
4. Zakharova, N.V., Kononikhin, A.S., Indeykina, M.I., Bugrova, A.E., Strelnikova, P., Pekov, S., Kozin, S.A., Popov, I.A., Mitkevich, V., and Makarov, A.A. (2022). Mass Spectrometric Studies of the Variety of Beta-Amyloid Proteoforms in Alzheimer’s Disease. Mass Spectrom. Rev., e21775.
5. Extracellular Phosphorylation of the Amyloid β 2-Peptide Promotes Formation of Toxic Aggregates during the Pathogenesis of Alzheimer’s Disease;Kumar;EMBO J.,2011
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