Affiliation:
1. Laboratory of Protein Based Functional Materials of Shanxi Province, Taiyuan Normal University, Jinzhong 030619, China
2. Department of Chemistry, Taiyuan Normal University, Jinzhong 030619, China
Abstract
Biomacromolecular probes have been extensively employed in the detection of metal ions for their prominent biocompatibility, water solubility, high selectivity, and easy modification of fluorescent groups. In this study, a fluorescent probe FP was constructed. The probe FP exhibited high specificity recognition for Cu2+. With the combination of Cu2+, the probe was subjected to fluorescence quenching. The research suggested that the probe FP carried out the highly sensitive detection of Cu2+ with detection limits of 1.7 nM. The fluorescence quenching of fluorescamine was induced by Cu2+ perhaps due to the PET (photoinduced electron transfer) mechanism. The FP-Cu2+ complex shows weak fluorescence, which is likely due to the PET quenching effect from Cu2+ to fluorescamine fluorophore. Moreover, the probe FP can be employed for imaging Cu2+ in living cells. The new fluorescent probe developed in this study shows the advantages of good biocompatibility and low cytotoxicity. It can be adopted for the targeted detection of Cu2+ in cells, and it has promising applications in the mechanism research and diagnosis of Cu2+-associated diseases.
Funder
Research Fund of Science and Technology Innovation Platform of Shanxi Province
National Natural Science Foundation of PR China
Scientific and Technological Innovation Programs and the Teaching Reform Innovation Programs of Higher Education Institutions in Shanxi
Shanxi Province College Student Innovation and Entrepreneurship Plan Training Project