Nanofibrous ε-Polycaprolactone Matrices Containing Nano-Hydroxyapatite and Humulus lupulus L. Extract: Physicochemical and Biological Characterization for Oral Applications

Author:

Villanueva-Lumbreras Jaime1,Rodriguez Ciro23ORCID,Aguilar María Rosa45ORCID,Avilés-Arnaut Hamlet1,Cordell Geoffrey A.67ORCID,Rodriguez-Garcia Aida12ORCID

Affiliation:

1. Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Instituto de Biotecnología, Ciudad Universitaria, Ave. Pedro de Alba S/N, San Nicolás de los Garza 66455, NL, Mexico

2. Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Ave. Eugenio Garza Sada 2501, Monterrey 64849, NL, Mexico

3. Laboratorio Nacional de Manufactura Aditiva y Digital (MADIT), Apodaca 66629, NL, Mexico

4. Instituto de Ciencia y Tecnología de Polímeros (ICTP-CSIC), 28006 Madrid, Spain

5. Networking Biomedical Research Centre in Bioengineering, Biomaterials and Nanomedicine, CIBER.BBN, 28029 Madrid, Spain

6. Natural Products Inc., Evanston, IL 60201, USA

7. College of Pharmacy, University of Florida, Gainesville, FL 32610, USA

Abstract

Oral bone defects occur as a result of trauma, cancer, infections, periodontal diseases, and caries. Autogenic and allogenic grafts are the gold standard used to treat and regenerate damaged or defective bone segments. However, these materials do not possess the antimicrobial properties necessary to inhibit the invasion of the numerous deleterious pathogens present in the oral microbiota. In the present study, poly(ε-caprolactone) (PCL), nano-hydroxyapatite (nHAp), and a commercial extract of Humulus lupulus L. (hops) were electrospun into polymeric matrices to assess their potential for drug delivery and bone regeneration. The fabricated matrices were analyzed using scanning electron microscopy (SEM), tensile analysis, thermogravimetric analysis (TGA), FTIR assay, and in vitro hydrolytic degradation. The antimicrobial properties were evaluated against the oral pathogens Streptococcus mutans, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans. The cytocompatibility was proved using the MTT assay. SEM analysis established the nanostructured matrices present in the three-dimensional interconnected network. The present research provides new information about the interaction of natural compounds with ceramic and polymeric biomaterials. The hop extract and other natural or synthetic medicinal agents can be effectively loaded into PCL fibers and have the potential to be used in oral applications.

Funder

Universidad Autónoma de Nuevo León

National Council for Science and Technology

MICINN

Nanomedicine CSIC HUB

Publisher

MDPI AG

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