Evaluation of Biofilm Production and Antifungal Susceptibility to Fluconazole in Clinical Isolates of Candida spp. in Both Planktonic and Biofilm Form
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Published:2024-01-12
Issue:1
Volume:12
Page:153
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ISSN:2076-2607
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Container-title:Microorganisms
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language:en
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Short-container-title:Microorganisms
Author:
Marzucco Anna12ORCID, Gatti Giulia3ORCID, Montanari Maria Sofia1, Fantini Michela4, Colosimo Claudia2ORCID, Tamburini Maria Vittoria1, Arfilli Valentina1, Morotti Manuela1, Schiavone Pasqualina1, Congestrì Francesco1, Manera Martina1, Denicolò Agnese1, Brandolini Martina2, Taddei Francesca1, Grumiro Laura1ORCID, Zannoli Silvia1, Dirani Giorgio1ORCID, De Pascali Alessandra Mistral2ORCID, Sambri Vittorio12ORCID, Cricca Monica12ORCID
Affiliation:
1. Unit of Microbiology, The Great Romagna Hub Laboratory, 47522 Pievesestina, Italy 2. Department of Medical and Surgical Sciences—DIMEC, Alma Mater Studiorum—University of Bologna, 40126 Bologna, Italy 3. DIN—Department of Industrial Engineering, Alma Mater Studiorum—University of Bologna, 40126 Bologna, Italy 4. Health Services Research, Evaluation and Policy Unit, AUSL Romagna, 42123 Rimini, Italy
Abstract
Candida spp. are an important opportunistic pathogen that can represent a possible cause of severe infections, especially in immunocompromised individuals. The clinical impact of Candida spp. depends, in part, on the ability to form biofilms, communities of nestled cells into the extracellular matrix. In this study, we compared the biofilm formation ability of 83 strains of Candida spp. isolated from blood cultures and other materials, such as respiratory samples, urine, and exudate, and their sensitivity to fluconazole (FLZ). Strains were divided into tertiles to establish cut-offs to classify isolates as low, moderate, or high biofilm producers (<0.26, 0.266–0.839, >0.839) and biofilms with low, moderate, or high metabolic activity (<0.053, 0.053–0.183, >0.183). A non-linear relationship between biofilm production and metabolic activity was found in C. glabrata and C. tropicalis. In addition, the increase in minimum biofilm eradication concentrations (MBEC50) compared to the Minor Inhibitory Concentration (PMIC) of the planktonic form in Candida spp. confirms the role of biofilm in the induction of resistance to FLZ.
Subject
Virology,Microbiology (medical),Microbiology
Reference20 articles.
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