Isolation and Characterization of Plant-Growth-Promoting Bacteria Associated with Salvinia auriculata Aublet-Reference-Cited by-同舟云学术

Isolation and Characterization of Plant-Growth-Promoting Bacteria Associated with Salvinia auriculata Aublet

Published:2024-09-06 Issue:9 Volume:12 Page:1842
ISSN:2076-2607
Container-title:Microorganisms
language:en
Short-container-title:Microorganisms

Author:

Goulart Jussara Tamires de Souza Silva¹,Quintanilha-Peixoto Gabriel²^ORCID,Esteves Bruno dos Santos³,de Souza Suzane Ariadina⁴,Lopes Pollyanna Santiago⁵,da Silva Nathália Duarte⁵,Soares Julia Ribeiro¹,Barroso Laura Mathias¹,Suzuki Marina Satika³,Intorne Aline Chaves¹⁶

Affiliation:

1. Laboratory of Physiology and Biochemistry of Microorganisms, State University of Northern Rio de Janeiro—UENF, Campos dos Goytacazes 28013-602, RJ, Brazil

2. Laboratory of Function and Chemistry of Proteins and Peptides, State University of Northern Rio de Janeiro—UENF, Campos dos Goytacazes 28013-602, RJ, Brazil

3. Laboratory of Environmental Sciences (LCA), State University of Northern Rio de Janeiro—UENF, Campos dos Goytacazes 28013-602, RJ, Brazil

4. Laboratory of Biotechnology, State University of Northern Rio de Janeiro—UENF, Campos dos Goytacazes 28013-602, RJ, Brazil

5. Laboratory of Cell and Tissue Biology, State University of Northern Rio de Janeiro—UENF, Campos dos Goytacazes 28013-602, RJ, Brazil

6. Laboratory of Chemistry and Biology, Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro, Volta Redonda 27213-100, RJ, Brazil

Abstract

Salvinia auriculata Aublet is a floating aquatic plant, capable of absorbing the excess of nutrients and water contaminants and can be used in effluent treatment plants. The ability to survive in degraded areas may be related to the association with beneficial bacteria capable of promoting plant growth. However, little is known about the microbiota associated with this aquatic plant and its potential application to the aquatic environment. In this sense, this work aims to identify bacteria associated with S. auriculata that could be able to promote plant growth. Eighteen bacterial strains were identified by sequencing of the 16S rRNA gene, belonging to the genera Agrobacterium, Bacillus, Curtobacterium, Enterobacter, Pseudomonas, Siccibacter, and Stenotrophomonas. All isolates produced indole compounds, 12 fixed N2, and 16 solubilized phosphate. A new strain of Enterobacter (sp 3.1.3.0.X.18) was selected for inoculation into S. auriculata. For this purpose, 500 mL of nutrient solution and 1 g of the plant were used in the control and inoculated conditions. Enterobacter inoculation promoted a significant increase (p ≤ 0.05) in fresh plant biomass (17%) after 4 days of cultivation. In summary, the present study characterized 18 plant-growth-promoting bacteria isolated from S. auriculata with potential for biotechnological application, such as the production of bioinoculants or biomass resources, to protect or improve plant growth under conditions of stress.

Funder

Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro

Publisher

MDPI AG

Link

https://www.mdpi.com/2076-2607/12/9/1842/pdf

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